医用生物力学杂志

Mechanical Properties of Cells Estimated by Different Models of Micropipette Aspiration

关键词: micropipette  aspiration  mechanical  models  properties  compressibility  of  cell  dimension  

面向3D打印植介入体的生物力学设计

关键词: 增材制造  微孔结构  力学设计  粉末材料  力学问题  

医用植介入体至少包含两类重要的生物力学问题:一是其自身结构的强度等生物力学问题,二是与宿主之间的相互作用;二者对于其成败都非常重要。增材制造技术(3D打印)为个性化植入假体的应用推广带来希望,个性化植入假体具有巨大的临床和市场需求。然而,个性化植入假体设计与临床应用仍有亟待解决的科学和关键技术问题,现有的临床案例主要考虑基于解...

Molecular and Cellular Immuno-Engineering

关键词: molecular  and  cellular  

Genetically-encoded biosensors based on fluorescence proteins(FPs)and fluorescence resonance energy transfer(FRET)have enabled the specific targeting and visualization of signaling events in live cells with high spatiotemporal resolutions.Single-molecule FRET biosensors have been successfully developed to monitor the...

Organization and Ultra-Structural Components of Endothelial Surface Glycocalyx Revealed by Stochastic Optical Reconstruction Microscopy(STORM)

关键词: organization  components  endothelial  surface  glycocalyx  revealed  optical  reconstruction  microscopy  storm  

Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the homeostasis of the circulatory system.In addition to forming a transport barrier between the blood and v...

血流动力学调控血管重塑依赖性的斑马鱼尾部静脉血管的形成

关键词: 静脉血管  流动力学  斑马鱼  

血管新生是指血管内皮细胞在原有的血管基础上通过出芽、迁移和增殖等方式形成新的毛细血管网络。随后,为了进一步适应组织器官的氧和营养的需要,整个血管网络会进行血管重塑,在此过程中会选择性地修剪一些局部的毛细血管,进而成熟为具有明确分级的功能化的血管网络结构,这一过程叫做血管修剪(vascular pruning)[1-2]。越来越多的文献报道血流动...

Mechanosensing via Immunereceptors

关键词: mechanosensing  immunereceptors  

The immune response is orchestrated by a variety of immune cells,the function of which then is determined by the collective signals from different immunoreceptors.Recent studies have highlighted the presence of mechanical force on these receptor-ligand pairs and its important role in regulating antigen recognition/di...

Nuclear Mechanotransduction in Cellular Mechanobiology and Molecular Mechanomedicine

关键词: nuclear  mechanotransduction  cellular  mechanobiology  molecular  mechanomedicine  

It is known that mechanical forces play critical roles in physiology and diseases but the underlying mechanisms remain largely unknown[1].Most studies on the role of forces focus on cell surface molecules and cytoplasmic proteins.However,increasing evidence suggests that nuclear mechanotransduction impacts nuclear ac...

How does Mechanical Loading Make Bone Microstructure: Modeling vs. Remodeling

关键词: mechanical  loading  make  bone  microstructure  modeling  vs  remodeling  

Bone modeling and remodeling are governed by distinct biochemical processes that may hold unique opportunities for optimizing bone mass[1,2].Remodeling refers to the coupled resorption and subsequent formation on the bone surface,while modeling represents uncoupled formation or resorption.Mechanical loading is known ...

Endocytosis and Vesicular Transport of Plasmid DNA in Cells During Electric Field-Mediated Gene Delivery

关键词: endocytosis  vesicular  transport  plasmid  dna  cells  during  electric  gene  delivery  

Pulsed electric field has been used widely as a nonviral approach to improving gene delivery in basic and translational research[1-2].The technique has been called electrotransfection(ET),electroporation,electrogene transfer,and gene electroinjection in the literature [1,3].It has a great potential to improve clinica...

Effect of Biophysical Cues on Cell Reprogramming

关键词: effect  biophysical  cues  cell  reprogramming  

Cell reprograming technologies have broad applications in cell therapy,disease modeling and drug screening.Direct reprogramming is the process of converting from one cell type into a very distantly related cell type.In this direct conversion process,cells do not proceed through a pluripotent stage,which can be time-c...

Dynamics of Collective Cells

关键词: dynamics  collective  cells  

The dynamic behaviors of collective cells play a significant role in many physiological and pathological processes,e.g.,embryonic development and cancer metastasis.In this talk,theoretical models,numerical simulations,and experimental measurements are combined to investigate the dynamics of collective cells[1-5].Firs...

Mechanical Regulation of Calcium Response and Trail Formation of Neutrophils on Endothelium

关键词: mechanical  regulation  of  calcium  response  and  trail  formation  neutrophils  on  endothelium  

Atherosclerosis or fibrosis and cirrhosis undergo chronic inflammation associated with the adhesion between neutrophils and endothelial cells(ECs)that is mediated by their respective cellular adhesive molecules on stiffened blood vessel wall or extracellular matrix(ECM)under shear flow[1-3].However,the mechanical dep...

MRI-Based Patient-Specific Left Ventricular Model to Determine the Effects of Trabeculae Carneae on the Diastolic and Systolic Functions

关键词: ef  trabeculae  carneae  the  diastolic  and  systolic  f  model  

Introduction Trabeculae carneae are irregular structures that cover the endocardial surfaces of both ventricles of human heart and account for a significant portion of the ventricular mass.However,the role of trabeculae carneae in left ventricular(LV)function is not well understood.Previous reports suggested that tra...

The Role of Non-Coding RNA in Vascular Remodeling Induced by Mechanical Stress

关键词: rna  vascular  remodeling  induced  mechanical  stress  

Vascular remodeling is the essential pathogenic process of various cardiovascular disorders,including hypertension,atherosclerosis,stroke,and restenosis after vein graft.The main characterization of vascular remodeling is abnormal variations of vascular cell phenotype,morphological structure and functions such as mig...

3D Bio-Printed Glioblastoma-Vascular Niche Models

关键词: gbm  niche  models  

Introduction Glioblastoma multiforme(GBM),a malignant brain tumor,is highly invasive and use brain microvessels to migrate and invade.Studying the perivascular invasion/migration of GBM may enable new possibilities in GBM therapy.However,the lack proper 3D study models that recapitulate GBM hallmarks restricts invest...

Mechanical Force Drives the Polarization and Orientation of Collective Cells

关键词: mechanical  force  the  polarization  orientation  collective  cells  

Collective cell groups are organized to form specific patterns that play an important role in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to the behaviors of single cells that have been studied intensively from many aspects(cell migration,ad...

Synthetic Human Embryo-Like Structure: A New Paradigm for Human Embryology

关键词: a  new  paradigm  for  human  embryology  synthetic  structure  

Early human embryonic development remains mysterious due to drastic species divergences between humans and othermammalian models and limited accessibility to human embryo samples. Recent studies from my laboratory and others haveshown that under suitable culture conditions human pluripotent stem cells ( hPSCs) can un...

响应性纳米药物递送系统构建及肿瘤治疗研究

关键词: 纳米药物递送系统  ph  溶酶体逃逸  电荷反转  响应性  靶向分子  细胞摄取  双靶向  肿瘤生长  纳米颗粒  控释系统  细胞凋亡  肿瘤治疗  

背景恶性肿瘤是威胁人类健康和生命的重大疾病之一。传统临床手术、放疗及化疗治疗手段仍存在诸多缺陷,如易复发、无靶向特异性、多药耐药性及严重毒副作用等。纳米颗粒药物载体由于其独特的增强渗透性和滞留性(EPR)效应,在提高抗肿瘤药物生物利用率、增强疗效以及减少毒副作用方面发挥着重要作用[1]。智能药物递送系统一般以纳米颗粒为药物载体...

Combined OVX and Concurrent Mechanical Disuse Induced Osteocytes Morphological Alteration, and Mitigation by Mechanobiology and Sclerostin Antibody

关键词: ovx  hls  mitigation  by  mechanobiology  sclerostin  antibody  combined  and  concurrent  mechanical  disuse  induced  osteocytes  morphological  alteration  

Osteoporosis and osteopenia are major health issues that mainly affect elderly people,women after menopause and immobilized patients.Our previous studies have proved that sclerostin antibody(Scl-Ab)can dramatically enhance bone formation and reduce bone resorption in a severe osteoporosis rat model with the combinati...

基于临床QCT影像的机器学习方法预测骨强度

关键词: 骨强度  qct  机器学习模型  机器学习方法  

当今人口老龄化现象日益显著,骨质疏松、骨质增生等骨退变疾病是中老年群体的常见病、多发病。2018年10月国家卫健委的调查结果显示,我国65岁以上人群骨质疏松症患病率高达32.0%;50岁以上人群低骨量率为46.4%。骨质疏松最严重的后果是骨折。骨折的原因是骨的强度/刚度降低,不足以承担外部载荷。临床上常用的评价骨强度的方法是双能X线骨密度仪测...

Bone Mechanobiology On-a-Chip

关键词: bone  mechanobiology  mlo  

Bone is able to adapt its composition and structure in order to suit its mechanical environment.Osteocytes,bone cells embedded in the calcified matrix,are believed to be the mechanosensors and responsible for orchestrating the bone remodeling process[1].However,detailed cellular and molecular mechanism underlying ost...

Systems Immunology Enabled Immune Engineering

关键词: systems  immunology  enabled  immune  engineering  

The immune checkpoint blockade has revolutionized cancer treatment.However,not all cancer types are susceptible to this therapy.Even in melanoma,one of the best scenario,about half of the patients do not respond to immune checkpoint blockade.Since CD8+T cell is the main driving force behind cancer elimination,then ha...

Engineering of Cell Spatiotemporal Mechanical Microenvironment

关键词: engineering  cell  spatiotemporal  mechanical  microenvironment  

Cells in vivo are in a three-dimensional(3D)complicated microenvironment composed of various biological,physical and chemical cues.Although it is well accepted that biological and chemical cues can significantly influence cell functions,more and more evidence has also shown that physical cues are also vital.Using tod...

机械力诱导MHC-Ⅰ构象变化增强TCR抗原识别及T细胞活化

关键词: tcr  刺激性  机械力  细胞活化  抗原识别  构象变化  mhc  

CD8+T细胞主要通过T细胞表面受体(T cell recep tor,TCR)识别并与Ⅰ型主要组织性复合物提呈的抗原(pMHC-Ⅰ)相互作用[1]。TCR对刺激型抗原的识别在CD8+T细胞毒性和适应性免疫中发挥着关键作用。许多证据表明机械力可以延长TCR与刺激性pMHC作用的键合时间(bond lifetime)形成抗原特异性逆锁键(catch bond)[2],并且这种逆锁键对抗原识别非常重要。...

Surface Topography Modulates Cell Mechanosensing of Extracellular Matrix

关键词: surface  topography  modulates  cell  mechanosensing  of  extracellular  matrix  

The interaction between the cell membrane and the extracellular matrix is crucial for many cellular functions by modulating mechanosensitive signaling pathways.Physical properties of the extracellular matrix such as stiffness and topography affect such interactions.Our recent work reveals that surface topography of t...

Engineering Cells and Tissue for Studying Development and Disease

关键词: engineering  cells  tissue  for  studying  development  disease  

Cell and Tissue Engineering provides exciting opportunities for studying development and Disease.In this talk,we will focus on cell chirality,also known as handedness and left-right(LR)asymmetry,which is an intrinsic capability of the cell telling left from right(1)The development of the vertebrate body plan with lef...

Ex Vivo Dynamics of Human Glioblastoma Cells in a Microvasculature-on-a-Chip System Correlates with Tumor Heterogeneity and Subtypes

关键词: ex  vivo  dynamics  human  glioblastoma  cells  a  system  subtypes  

The brain tumor perivascular niche(PVN),the region in the vicinity of microvessels is a prime location for brain tumor stem-like cells(BTSCs)[1].Tumor microvasculature creates a complex microenvironment consisting of various cell types,the extracellular matrix,and soluble factors that mediate cell-cell interaction.Th...

冠脉循环和心肌力学性能

关键词: 心肌力学  高血压  小静脉  小动脉  冠脉循环  冠状动脉粥样硬化  心血管疾病  应变能函数  

心血管疾病是危害人类生命健康最严重的疾病之一,其巨额的防治费用成为家庭、社会和国家的沉重负担。心血管生物力学领域的研究不仅对于揭示正常血液循环的机理,认识血管生长、衰老的自然规律,而且对于阐明心血管疾病的发病机理以及提供诊断与防治的一些基本原理包括心血管新型药物和新技术的研发都将有重要的理论和实际意义。我课题组的研究方...

Perlecan/Hspg2 Deficiency Impairs Bone’s Calcium Signaling and Associated Transcriptome in Response to Mechanical Loading

关键词: calcium  signaling  mechanical  loading  

Perlecan,a heparan sulfate proteoglycan,acts as a mechanical sensor for bone to detect external loading.Deficiency of perlecan increases the risk of osteoporosis in patients with Schwartz-Jampel Syndrome(SJS)and attenuates loading4nduced bone formation in perlecan deficient mice(Hypo).Considering that intracellular c...

细胞体积调控的力学生物学研究

关键词: 细胞体积  压力调控  力学性质  收缩力  细胞骨架  外力作用  力学生物学  

水是生命之源,细胞内部的各种生化过程都离不开水分子的参与,而细胞体积的变化会直接改变细胞内水分子含量,进而影响细胞内蛋白质的水化。蛋白质以及与蛋白质结合的水分子占据了很大一部分细胞内空间,留给自由水分子的空间是非常有限的。因此,即便是很小比例的细胞内水分子的损失或增加也会极大的影响蛋白质功能和细胞的正常活动。细胞体积调控...

Ultrasound Tweezers Trigger Single-Cell Mechanical Dynamics for Cell Mechanophenotyping

关键词: aaa  ultrasound  tweezers  trigger  mechanical  dynamics  cell  mechanophenotyping  

Cells actively modulate mechanobiological circuitry against external perturbations to stabilize whole cell/tissue physiology.The dynamic adaption of cells to mechanical force is critical for cells to perform vital biological functions,from single cell migration to embryonic development.Dysregulation of such dynamics ...

力学修饰三维脱细胞骨支架促进大块骨缺损修复及其力学生物学机制

关键词: 骨缺损修复  内源性  骨组织工程支架  力学生物学  骨支架  

骨是除了皮肤和血液之外的第三大移植组织类型,由外伤引起的骨缺损以及由疾病等造成的大块骨切除均需要使用大量的骨移植物。尽管骨组织工程和再生医学等概念的提出为大块骨缺损提供了诸多很有前景的修复方法,但大块骨缺损的医治至今仍未得到有效解决,大块骨损伤的重建和修复仍是骨修复领域的研究难点。作者课题组构建了多种微结构一致而基质刚...

心血管植介入体的生物力学分析和仿生设计

关键词: 生物力学分析  微环境  心血管  细胞存活率  支架植入  剪切率  结构和功能  仿生设计  

植介入手术是临床治疗心血管疾病的最重要手段之一。然而,血栓形成和血管内膜增生而致的再狭窄限制了血管支架、人造血管、机械瓣膜等植介入体的手术效果。尽管这一系列事件的相关机理并不十分清楚,但是血流动力学因素在其中起着关键的作用已成为不争的事实。为此,开展植介入体与血液和血管组织之间相互作用的研究无疑将利于临床手术方案的制定...

抗血管生成致内皮细胞释放外泌体促进肿瘤血管形成

关键词: 肿瘤血管形成  肿瘤生长  抗血管生成  外泌体  内皮细胞  

抗血管生成疗法(anti-angiogenic therapies,AATs)被用于多种恶性癌症的治疗,其临床疗效因继发性的肿瘤血管形成(vasculogenesis)和肿瘤生长而受限[1-3]。本研究试图揭示AATs治疗后肿瘤血管生成和肿瘤生长的分子机制。首先,将微小RNA-9(microRN A-9,miR-9)转染至人脐静脉内皮细胞,以模拟肝癌中的肿瘤相关性内皮细胞[4-5],探讨AATs对内皮细胞的影...

ANTXR1在压力促BMSCs细胞膜片成软骨响应中作用的研究

关键词: 骨髓间充质干细胞  压力  炭疽毒素受体蛋白1  成软骨  

目的寻找修复关节软骨的方法一直是临床难题,近年来软骨组织工程研究成为一个新的突破口,大量研究表明适宜的力学刺激可以促进BMSCs向成软骨方向分化,为了研究其中的力学信号转导机制,本实验将探究与整合素有很多相似性的Ⅰ型跨膜蛋白ANTXR1在压力促BMSCs细胞膜片成软骨分化中所发挥的作用。方法首先对BMSCs细胞膜片加以压力刺激,检测ANTXR1、In...

Irisin Attenuates Osteoarthritis by Inhibiting Apoptosis of Osteocytes Through Activating Erk Signaling Pathway

关键词: irisin  attenuates  osteoarthritis  inhibiting  apoptosis  osteocytes  activating  erk  signaling  pathway  

Osteoarthritis(OA)is an inflammatory disease involving the joints that is prevalent in the global aging population.The purpose of this study is to determine whether irisin can attenuate osteoarthritis(OA)progression in anterior cruciate ligament transection(ACLT)mice models and the mechanism of irisin therapy effect ...

细胞外基质硬度和炎症微环境的协同作用对间充质干细胞成骨分化影响的力学生物学机制

关键词: 间充质干细胞  基质硬度  成骨分化  信号通路  

背景间充质干细胞(MSCs)所处微环境非常复杂,现有研究多是考虑单一力学或生化因素影响,对于力学、生化因子的协同作用对MSCs分化行为的影响还缺乏系统认识。目的研究细胞外基质硬度与炎症因子(IL-1β)的协同作用对MSCs成骨分化的影响及其可能的力学生物学机制。方法利用不同浓度的GelMA构建不同硬度(26、60、88 kPa)的细胞培养基底,以模拟不同的...

基质弹性通过整合素/FAK触发p53核内聚集影响软骨细胞功能及骨关节炎发病

关键词: 基质弹性  p53  软骨细胞  

随着社会老龄化及全民健身的普及,骨性关节炎(OA)的发病增多,其机理及治疗手段越发引起关注。体内、体外实验表明,OA的发病与力学环境的变化密切相关,力学环境影响了关节软骨细胞功能、表型维持等一系列重要的生命活动。本课题组利用压痕实验测定小鼠正常关节软骨弹性模量,并使用包裹胶原的聚丙烯酰胺凝胶制备弹性模量E=105 Pa的硬基质来模拟正...

一种对抗骨骼肌萎缩的新型力学疗法及其机制

关键词: 力学疗法  废用性肌萎缩  磁性水凝胶  

背景废用性肌肉萎缩是指由活动丧失,活动受限或太空飞行引起的骨骼肌功能、结构、生化标志物的一系列变化的一种骨骼肌疾病。主要临床症状是肌肉体积缩小,肌肉重量减轻,肌纤维的超微结构变得更薄甚至消失。传统的物理疗法如按摩等无法为患者提供精准的力学拉伸和应变,因此仍然迫切需要一种更为准确的治疗手段。随着生物力学的发展,人们对组织和...

体温环境下腰椎间盘疲劳损伤的力学性能研究

关键词: 椎间盘  疲劳损伤  力学性能  

目的腰椎间盘退变是导致椎间盘突出症、引起腰痛的病理基础,是骨科临床常见的一种疾病。腰椎间盘退变的病因是复杂的,其中长期力学载荷积累和载荷突变被认为是导致退变的重要原因。在疲劳载荷作用下腰椎间盘会出现疲劳损伤,常见于运动员长跑、新兵训练以及老年人的日常活动,主要表现为显微裂纹萌生及扩展、力学性能下降等,危害很大。本文自行开...